- Science
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Light Source and Detector Options 7 min
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Stripping the Membrane 3 min
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Re-probing and Re-imaging 2 min
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Troubleshooting 9 min
- Best Practices
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Imaging a Wet vs. Dry Membrane 5 min
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Image Manipulations and Storage Considerations 4 min
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Stripping Your Membrane 3 min
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Reprobing Your Membrane 2 min
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Troubleshooting Your Membrane 3 min
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General Best Practices 4 min
- In the Lab
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Detection 2 min
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Knowledge Check
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Fluorescence: Detection
There are several things you need to keep in mind when imaging a fluorescent Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative.
There are several things you need to keep in mind when imaging a fluorescent Western blot. In this course, you'll learn about the differences of imaging a wet membrane versus a dry membrane. You'll also learn what stripping and reprobing is and how multiplexing is a more quantitative alternative.