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Are you analyzing the same samples under the same set of experimental conditions and still getting dissimilar results? Consistency in lab protocols and technique is key to reproducible research results. Get to know your blots better, so you know what to do when things go wrong.
Basics: Learn the foundational concepts of Western blotting including sample and gel preparation, and electrotransfer.
Chemiluminescence Detection: Learn best practices for blocking, antibody incubation, and detection using chemiluminescence.
Near-Infrared Detection: Learn best practices for blocking, antibody incubation, and detection using near-infrared fluorescence.
Learn how to perform serial dilutions, concentration calculations, and use proper control samples.
For more than 25 years, LI-COR has been developing and optimizing solutions for Western blot detection using Odyssey® Imaging Systems, IRDye® Infrared Dye fluorophores, and Image Studio™ software products. Connect with our experts through Lambda U and get all your Western blot questions answered.