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Importance of Estimating Protein Concentration 3 min
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What is a Protein Assay? 4 min
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Science Behind Protein Assays 3 min
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Spectrophotometry 3 min
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Ultraviolet Absorption Spectroscopy 3 min
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Bradford Assay 5 min
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Lowry Assay 5 min
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BCA Assay 5 min
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Best Practices 4 min
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Next Steps 3 min
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Estimating Protein Concentration
Learn about the importance of estimating your protein concentration and how to incorporate this technique into your experimental design.
As scientists who perform Western blots all the time, we all know that the process takes time. So, why do we have to estimate our protein concentration before every Western blot? This course explores that question and more while going into detail on how protein concentration can affect your assay and even the conclusions that you draw from your results.